Composite
Part:BBa_K1660008:Design
Designed by: Dai Yuanyi Group: iGEM15_BNU-CHINA (2015-09-08)
J23100-B0034-bace16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 56
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 152
Illegal NgoMIV site found at 515
Illegal NgoMIV site found at 587
Illegal NgoMIV site found at 839
Illegal AgeI site found at 446 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add J23100, a constitutive promoter upstream the coding sequence of bace16, so that it can express Bace16.
Source
It comes from Bacillus nematocida.